Role of YopE and LcrH in effector translocation, HeLa cell cytotoxicity and virulence
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چکیده
........................................................................................... 5 PAPERS IN THIS THESIS.................................................................... 6 INTRODUCTION .................................................................................. 7 Yersinia spp. and their diseases..............................................................................7 Protective antigens and treatment of Yersinia infections .........................................................8 Virulence properties of Yersinia .............................................................................8 Motility.....................................................................................................................................8 Adhesion proteins.....................................................................................................................9 Capsule and LPS ......................................................................................................................9 Target cells and cellular model systems...............................................................10 Type III Secretion System .....................................................................................11 The Secretion Apparatus ........................................................................................................11 T3SS chaperones ....................................................................................................................12 The secretion signal................................................................................................................13 Substrate specificity switch....................................................................................................13 Translocation of effector proteins into eukaryotic cells.........................................................14 Regulation of the T3SS..........................................................................................15 Positive regulation of T3S......................................................................................................15 The Low calcium response.....................................................................................................15 De-repression of Yop synthesis by secretion of negative regulatory elements......................16 LcrH is a negative regulator in Yersinia.................................................................................17 Effector proteins of Yersinia .................................................................................18 The family of RhoGTPases ...................................................................................20 Regulation of RhoGTPases ....................................................................................................20 RhoGTPases and the actin cytoskeleton ................................................................................21 Control of endocytosis by RhoGTPases.................................................................................22 RhoGTPases as targets for bacterial toxins............................................................................23 GENERAL AND SPECIFIC AIMS .................................................... 24
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